In their experiments, the scientists engineered fibroblasts from a male fetus (XY) to produce a line of pluripotent stem cells. About 1% of these cells spontaneously lost the Y chromosome (remaining as X0) and were injected into blastocysts of a donor female mouse. The blastocysts were transplanted into surrogate mothers who gave birth to female mice with genetic material from the first male mouse . When these females mated with other males, offspring with genetic material were born from two male mice, that is, with two parents .