To understand the importance of his work and how it led to new sequencing techniques, we need to understand the limitations of the most popular genetic sequencing technique: PCR.
All the videos of the series in:
- About “Biocosas”:
Directed and presented by Pablo Barrecheguren (@pjbarrecheguren), "Biocosas" is a scientific dissemination project produced by Minifilms TV in collaboration with Muy Interesante and with the collaboration of the Spanish Foundation for Science and Technology – Ministry of Science and Innovation.
References:
Avila, J., Mayor, F., & Ruiz-Desviat, L. (2019). Margarita Salas (1938–2019). Nature, 576(7786), 208–208.
Brock, T.D., & Freeze, H. (1969). Thermus aquaticus gen. n. and sp. n., a Non-sporulating Extreme Thermophile. In JOURNAL OF BACTERIOLOGY (Vol. 98).
Ramesh, R. et al., (1992). Polymerase chain reaction. The National Medical Journal of India
Rooms, M. (2016). My scientific life. Bacteriophage, 6(4), e1271250.
Rooms, M. (2012). My life with bacteriophage φ In Journal of Biological Chemistry (Vol. 287, Issue 53, pp. 44568–44579).
Yao, K., González-Escalona, N., & Hoffmann, M. (2021). Multiple Displacement Amplification as a Solution for Low Copy Number Plasmid Sequencing. Frontiers in Microbiology , 12 (February).
